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PUBLICATION | Prof. Yuri Feldman

PUBLICATION

2021
K.A. Baksheeva, R.V. Ozhegov, , G.N. Goltsman, , N.V. Kinev, , V. P. Koshelets, , A. Kochnev, , Betzalel, N. , Puzenko, A. , Ben Ishai, P. , and Feldman., Y. . 3/5/2021. The Sub Thz Emission Of The Human Body Under Physiological Stress. Ieee Transactions On Terahertz Science And Technology , 11, 4, Pp. 381-388. Abstract

We present evidence that in the sub-THz frequency band, human skin can be considered as an electromagnetic bio-metamaterial, in that its natural emission is a product of skin tissue geometry and embedded structures. Radiometry was performed on 32 human subjects from 480 to 700 GHz. Concurrently, the subjects were exposed to stress, while heart pulse rate (PS) and galvanic skin response (GSR) were also measured. The results are substantially different from the expected black body radiation signal of the skin surface. PS and GSR correlate to the emissivity. Using a simulation model for the skin, we find that the sweat duct is a critical element. The simulated frequency spectra qualitatively match the measured emission spectra and show that our sub-THz emission is modulated by our level of mental stress. This opens avenues for the remote monitoring of the human state.

the_sub_thz_emission_of_teh_human_body_under_physiological_stress.pdf
2020
Y. Horowitz, Lifshitz, M. , Greenbaum, A.  , Feldman, Yu. , Sokolov, A. P. , and Golodnitsky, D.. 12/19/2020. Review&Mdash;Polymer/Ceramic Interface Barriers: The Fundamental Challenge For Advancing Composite S160514-19.Olid Electrolytes For Li-Ion Batteries. Journal Of The Electrochemical Society, 167, Pp. 160514-19. Abstract
Composite-solid electrolytes, in which ion-conducting polymers are combined with superionic ceramics, could revolutionize electrochemical-energy-storage devices enabling higher energy density, providing greater stability during operation and enhanced safety. However, the interfacial resistance between the ceramic and polymer phases strongly suppresses the ionic conductivity and presents the main obstacle to the use of these materials. Here, we emphasize the need for a distinct focus on reducing energy barriers to interfacial ion transport and improving the cation transference number. To achieve this goal, it is essential to develop a fundamental understanding of the parameters that influence the interfacial barriers to ion transport in composite electrolytes, and to understand the effect of the type of ceramic (“active” and “inert”) and its content on ion-transport phenomena. We suggest that adapting the polymer chemistry, mainly directed on polymerized ionic liquids, (PolyILs), and combined with functionalization of the surface of ceramic nanoparticles is a promising route for overcoming the high-energy-barrier challenge. Owing to high content of ion-conducting ceramics and high t+ of PolyILs, the fractional contribution of the migrating cationic species to the total ionic conductivity of polymer-in-ceramic electrolytes via an interfacial percolation path, will be close to unity, thus eliminating complications that might arise from emerging concentration gradients during the operation of solid-state batteries.
horowitz_2020_j._electrochem._soc._167_160514.pdf
A. Puzenko Yu. Poluektov A. Anashkina Irina Petrushanko S. Fenk A. Bogdanova Yu. Feldma and L. Latypova, G. Barshtein. 8/2020. Oxygenation State Of Hemoglobin Defines Dynamics Of Water Molecules In Its Vicinity&Rdquo; J. Chem. Phys. 153, 135101 (2020); Https://Doi.org/10.1063/5.0023945. Journal Of Chemical Physic, 153, Pp. 135101-9. Abstract

This study focuses on assessing the possible impact of changes in hemoglobin (Hb) oxygenation on the state of water in its hydration shell as it contributes to red blood cell deformability. Microwave Dielectric Spectroscopy (MDS) was used to monitor the changes in interactions between water molecules and Hb, the number of water molecules in the protein hydration shell, and the dynamics of pre-protein water in response to the transition of Hb from the tense (T) to the relaxed (R) state, and vice versa. Measurements were performed for Hb solutions of different concentrations (5 g/dl–30 g/dl) in phosphate-buffered saline buffer. Cole–Cole parameters of the main water relaxation peak in terms of interactions of water molecules (dipole–dipole/ionic dipole) during the oxygenation–deoxygenation cycle were used to analyze the obtained data. The water mobility—represented by α as a function of ln τ—differed dramatically between the R (oxygenated) state and the T (deoxygenated) state of Hb at physiologically relevant concentrations (30 g/dl–35 g/dl or 4.5 mM–5.5 mM). At these concentrations, oxygenated hemoglobin was characterized by substantially lower mobility of water in the hydration shell, measured as an increase in relaxation time, compared to deoxyhemoglobin. This change indicated an increase in red blood cell cytosolic viscosity when cells were oxygenated and a decrease in viscosity upon deoxygenation. Information provided by MDS on the intraerythrocytic water state of intact red blood cells reflects its interaction with all of the cytosolic components, making these measurements powerful predictors of the changes in the rheological properties of red blood cells, regardless of the cause.

oxygenation_state_of_hb_defines_dynamics_of_water.pdf
Larisa Latypova, Barshtein, Gregory , Arbell, Dan , and Feldman, Yuri . 7/2020. The Contribution Of Storage Medium And Membranes In The Microwave Dielectric Response Of Packed Red Blood Cells Suspension. Applied Sciences , 10, 5, Pp. 1702-9. doi:10.1016/j.carbpol.2019.115217. Abstract
During cold storage, packed red blood cells (PRBCs) undergo slow detrimental changes that are collectively termed storage lesion. The aging of the cells causes alterations in the composition of the storage‐medium in the PRBC unit. In this paper, we present the comparison of the dielectric response of water in the primary (fresh) storage medium (citrate phosphate dextrose adenine solution, CPDA‐1) versus the storage medium from three expired units of PRBCs. Dielectric response of the water molecules has been characterized by dielectric spectroscopy technique in the microwave frequency band (0.5–40 GHz). The dominant phenomenon is the significant increase of the dielectric strength and decrease the relaxation time τ for the samples of the stored medium in comparison with the fresh medium CPDA‐1. Furthermore, we demonstrated that removing the ghosts from PRBC hemolysate did not cause the alteration of the dielectric spectrum of water. Thus, the contribution associated with water located near the cell membrane can be neglected in microwave dielectric measurements.
applsci-10-01702_storage.pdf
L. Latypova, Puzenko, A. , Levy, E. , and Feldman, Yu. . 6/2020. Dielectric Spectra Broadening As A Signature For Dipole&Ndash;Matrix Interactions. V. Water In Protein Solutions. J. Chem. Phys. , 220, Pp. 135101-9. Abstract

In this paper, the fifth of our series focused on the dielectric spectrum symmetrical broadening of water, we consider the solutions of methemoglobin (MetHb) in pure water and in phosphate-buffered saline (PBS). The universal character of the Cole–Cole dielectric response, which reflects the interaction of water dipoles with solute molecules, was described in Paper I [E. Levy et al., J. Chem. Phys. 136, 114502 (2012)]. It enables the interpretation of the dielectric data of MetHb solutions in a unified manner using the previously developed 3D trajectory method driven by the protein concentration. It was shown that protein hydration is determined by the interaction of water dipoles with the charges and dipoles located on the rough surfaces of the protein macromolecules. In the case of the buffered solution, the transition from a dipole-charged to a dipole–dipole interaction with the protein concentration is observed {see Paper III [A. Puzenko et al., J. Chem. Phys. 137, 194502 (2012)]}. A new approach is proposed for evaluating the amount of hydration water molecules bounded to the macromolecule that takes into account the number of positive and negative charges on the protein’s surface. In the case of the MetHb solution in PBS, the hydration of the solvent ions and their interaction with charges on the protein’s surface are also taken into consideration. The difference in hydration between the two solutions of MetHb is discussed.

dielectric_spectra_broadening_as_a_signature_for_dipole-matrix_interactions._v._water_in_protein_solutions_2020.pdf
Airat A. Khamzin, Lunev, Ivan V. , Popov, Ivan I. , Anna M. Greenbaum, , and Feldman, Yuri D. . 3/6/2020. Mechanisms Of Dielectric Relaxation Of Hexagonal Ice. Rensit, 12, 1, Pp. 87-94. Abstract
The article presents the results of theoretical and experimental studies of the dielectric relaxation of hexagonal ice in a wide temperature range. The model of dielectric relaxation of ice was developed explaining the origin of the dynamic crossovers in relaxation time at high and low temperatures. The results of the model are in agreement with experimental studies and explain the irreproducibility of dielectric experiments at low temperatures.
ice_paper.pdf
2019
A. Caduff, Ben Ishai, P. , and Feldman, Y. . 11/25/2019. Continuous Noninvasive Glucose Monitoring; Water As A Relevant Marker Of Glucose Uptake In Vivo. Biophysical Reviews, 11, 6, Pp. 1017-1035. 10.1007/s12551-019-00601-7. Publisher's Version Abstract
With diabetes set to become the number 3 killer in the Western hemisphere and proportionally growing in other parts of the world, the subject of noninvasive monitoring of glucose dynamics in blood remains a “hot” topic, with the involvement of many groups worldwide. There is a plethora of techniques involved in this academic push, but the so-called multisensor system with an impedance-based core seems to feature increasingly strongly. However, the symmetrical structure of the glucose molecule and its shielding by the smaller dipoles of water would suggest that this option should be less enticing. Yet there is enough phenomenological evidence to suggest that impedance-based methods are truly sensitive to the biophysical effects of glucose variations in the blood. We have been trying to answer this very fundamental conundrum: “Why is impedance or dielectric spectroscopy sensitive to glucose concentration changes in the blood and why can this be done over a very broad frequency band, including microwaves?” The vistas for medical diagnostics are very enticing. There have been a significant number of papers published that look seriously at this problem. In this review, we want to summarize this body of research and the underlying mechanisms and propose a perspective toward utilizing the phenomena. It is our impression that the current world view on the dielectric response of glucose in solution, as outlined below, will support the further evolution and implementation toward practical noninvasive glucose monitoring solutions.
continuous_noninvasive_glucose_monitoring_water_as_a_relevant_marker_01.pdf
K. Sasaki, Popov, I. , and Feldman, Y. . 11/16/2019. Water In The Hydrated Protein Powders; Dynamic And Structure. Journal Of Chemical Physics, 150, 11, Pp. 204504-8. Abstract
It is not an understatement to say that the interplay between water and protein is a fundamental aspect of life. The vitality of an organism depends on the functionality of its biological machinery, and this, in turn, is mediated in water. Yet, we understand surprisingly little about the nature of the interface between bulk water and the protein. On the one hand, we know that the nature of the bulk water is dominated by the existence of H-bonding and H-bonded networks. On the other hand, the protein surface, where much of the bioactivity is centered, is a complex landscape of hydrophilic and hydrophobic elements. So how does the interface between these two entities work and how do they influence each other? The question is important because if one understands how a particular protein interface influences the dynamics of the water, it then becomes an easily accessible marker for similar behavior in other protein systems. The dielectric relaxation of hydrated proteins
with different structures, lysozyme, collagen, and phycocyanin, has been reviewed in this paper. The dynamics of hydrated water was analyzed in terms of orientation and the ionic defect migration model. This approach enables to characterize the microscopic relaxation mechanism of the dynamics of hydration water on the different structures of the protein. In addition, our model is also capable of characterizing not only hydrated proteins but also polymer-water systems.
dielectric_response_of_hydrated_water_as_a_structural_component_of_01.pdf
Ivan Lunev, Greenbaum (Gutina), Anna , Feldman, Yuri , Petrov, Vladimir , Kuznetsova, Nina , Averianova, Natalia , Makshakova, Olga , and Zuev, Yuriy . 2019. Dielectric Response Of Hydrated Water As A Structural Component Of Nanofibrillated Cellulose (Nfc) From Different Plant Sources. Carbohydrate Polymers, 225. doi:10.1016/j.carbpol.2019.115217. Abstract
The current work illuminates the interplay between nanofibrillated cellulose (NFC) films and hydrated water. The NFC films from three sources of technological importance, i.e. cotton, wood and flax, are compared. It is shown that cellulose materials present slight variations in supramolecular structure depending on the plant origin. The structural differences determine both quantity and state of the water adsorbed by cellulose. Dielectric spectroscopy was employed to study the state of hydrated water as a probe of both the overall and specific marks of NFCs' structure. The measurements, carried out in the wide frequency (10(-2)Hz -10(6)Hz) and temperature (123 K-293 K) ranges, revealed the formation of non-interactive water clusters at low water content. At high water content, additional states of water were identified: Water in saturated glass-forming solution and bulk. These water states were shown to be determined by the NFC's structure and morphology.
dielectric_response_of_hydrated_water_as_a_structural_component_of.pdf
2018
Y. Feldman and Ben Ishai, P. . 10/23/2018. Water In Heterogeneous Matterinterfacial Water&Mdash;From Non-Organic To Organicsystems. In Isema 10/23/2018. Lublin, Poland: IEEE. Abstract
Water is the universal solvent of nature. Does this imply, however, that its interaction with its environment is also universal in its character? We present evidence that the broadening of the dielectric spectra of water presents universal features of dipolar interactions with different types of matrixes. The state of water adsorbed in heterogeneous materials is
determined by various hydration centers of the inhomogeneous material (the matrix) and it is significantly different from the bulk. In both cases, the dielectric spectrum of water is
symmetrical and can be described by the Cole–Cole (CC) function. The phenomenological model that describes a physical mechanism of the dipole–matrix interaction in complex underlying the CC behavior has been applied to water adsorbed  in porous glasses clays and hydrated proteins
water_in_heterogeneous_matter_isema_2018.pdf
N. Betzalel, Ben Ishai, P. , and Feldman, Y. . 7/16/2018. The Human Skin As A Sub-Thz Receiver &Ndash; Does 5G Pose A Danger To It Or Not?. Environmental Research, 163, 4, Pp. 208-216. Abstract
In the interaction of microwave radiation and human beings, the skin is traditionally considered as just an absorbing sponge stratum filled with water. In previous works, we showed that this view is flawed when we demonstrated that the coiled portion of the sweat duct in upper skin layer is regarded as a helical antenna in the sub-THz band. Experimentally we showed that the reflectance of the human skin in the sub-THz region depends on the intensity of perspiration, i.e. sweat duct's conductivity, and correlates with levels of human stress (physical, mental and emotional). Later on, we detected circular dichroism in the reflectance from the skin, a signature of the axial mode of a helical antenna. The full ramifications of what these findings represent in the human condition are still unclear. We also revealed correlation of electrocardiography (ECG) parameters to the
sub-THz reflection coefficient of human skin. In a recent work, we developed a unique simulation tool of human skin, taking into account the skin multi-layer structure together with the helical segment of the sweat duct embedded in it. The presence of the sweat duct led to a high specific absorption rate (SAR) of the skin in extremely high frequency band. In this paper, we summarize the physical evidence for this phenomenon and consider its implication for the future exploitation of the electromagnetic spectrum by wireless communication. Starting from July 2016 the US Federal Communications Commission (FCC) has adopted new rules for wireless broadband operations above 24 GHz (5 G). This trend of exploitation is predicted to expand to higher frequencies in the sub-THz region. One must consider the implications of human immersion in the electromagnetic noise, caused by devices working at the very same frequencies as those, to which the sweat duct (as a helical antenna) is most attuned. We are raising a warning flag against the unrestricted use of sub-THz technologies for communication, before the possible consequences for public health are explored.
the_human_skin_as_a_sub-thz_receiver_-_does_5g_pose_a_danger_to_it_or_not.pdf
 A Kochnev, Betzalel, N. , Ben Ishai, P. , and Feldman, Yu.. 6/10/2018. Human Sweat Ducts As Helical Antennas In The Sub-Thz Frequency Range-An Overview. Terahertz Science And Technology, 11, 3, Pp. 43-56. Abstract
Detailed anatomical studies of the human skin using optical coherence tomography (OCT) have revealed that the morphological structure of our eccrine sweat ducts is remarkably helical. These findings have raised the hypothesis that human sweat ducts can be the biological equivalent of helical antennas and hence resemble their electromagnetic (EM) behavior by receiving signals in the sub-THz frequency range. Here we show how this hypothesis evolved and was experimentally tested over the recent years, driven by the prospect of developing remote sensors for obtaining information about our physiological and mental state, as well as better understanding the consequences of using this frequency band for communications in the very near future.
tst_2018.pdf
2017
M. David, Levy, E. , Feldman, Y. , Ben Ishai, P. , Zelig, O. , Yedgar, S. , and Barshtein, G. . 8/2/2017. The Dielectric Spectroscopy Of Human Red Blood Cells: The Differentiation Of Old From Fresh Cells. Physiological Measurement, 38, 9, Pp. 1335-1348. Abstract
Objective: The objective of the study was to gauge the effect of storage lesions on the dielectric response of red blood cells (RBC), in particular those processes linked to deformations of the cellular membrane known as the β-dispersion. Approach: The dielectric response of RBC suspensions, exposed to blood-bank cold storage, was studied using time-domain dielectric spectroscopy (TDDS) in the frequency range of 500 kHz up to 1 GHz. The measured dielectric processes are characterized by their dielectric strength (Δε) and relaxation time (τ). Changes in the dielectric properties of the RBC suspensions due to storage-related lesions were evaluated. For a quantitative characterization of RBC lesions, we measured the deformability of fresh and stored RBC as expressed by their elongation
ratio (ER), which was achieved under a shear stress of 3.0 Pa. Main Result: The results show that the storage of RBC induced a statistically significant decrease of dielectric relaxation times. In addition, a sound correlation between the mean values of ER and the relaxation times was observed (Spearman’s correlation coefficient ρ = 0.847). We draw the conclusion that those alterations in the relaxation time are induced by changes in the shape of the RBC that happen during cold-storage. Significance: The evolution of the β-dispersion of RBC opens new possibilities in the blood bank inventory management.
david_2017_physiol._meas._38_1335_01.pdf
Yael Kurzweil-Segev, Popov, Ivan , Eisenberg, Ido , Yochelis, Shira , Keren, Nir , Paltiel, Yossi , and Feldman, Yuri . 2017. Confined Water Dynamics In A Hydrated Photosynthetic Pigment-Protein Complex. Physical Chemistry Chemical Physics, 19, 41, Pp. 28063-28070. doi:10.1039/c7cp05417c. Abstract
Water is of fundamental importance for life. It plays a critical role in all biological systems. In phycocyanin, a pigment-protein complex, the hydration level influences its absorption spectrum. However, there is currently a gap in the understanding of how protein interfaces affect water's structure and properties. This work presents combined dielectric and calorimetric measurements of hydrated phycocyanin with different levels of hydration in a broad temperature interval. Based on the dielectric and calorimetric tests, it was shown that two types of water exist in the phycocyanin hydration shell. One is confined water localized inside the phycocyanin ring and the second is the water that is embedded in the protein structure and participates in the protein solvation. The water confined in the phycocyanin ring melts at the temperature 195 +/- 3 K and plays a role in the solvation at higher temperatures. Moreover, the dynamics of all types of water was found to be effected by the presence of the ionic buffer.
Y. Kurzweil-Segev, Popov, Ivan , Solomonov, Inna , Sagi, Irit , and Feldman, Yuri . 2017. Dielectric Relaxation Of Hydration Water In Native Collagen Fibrils. Journal Of Physical Chemistry B, 121, 21, Pp. 5340-5346. doi:10.1021/acs.jpcb.7b02404. Abstract
The dielectric relaxation of hydrated collagen powders was studied over a wide temperature and frequency-range We revealed two mechanisms of dielectric relaxation in hydration water that are driven by the migration of ionic and orientation defects. At high water fractions in powders (h > 0.2), the hydration shell around the collagen triple helixes presents a spatial H-bonded network consisting of structural water bridges and cleft water channels. These two water phases, provide the long-range paths for proton hopping and orientation defect migration. At low water fractions (h < 0.2) and in the hydrated collagen samples after the dehydrothermal treatment, the hydration shell presents localized individual water compartments not connected to one another. In these cases, the relaxation mechanism due to proton hopping either disappears or becomes inhibited by the orientation defect migration.
Daniel Agranovich, Polygalov, Eugene , Popov, Ivan , Ben Ishai, Paul , and Feldman, Yuri . 2017. Inductive Dielectric Analyzer. Measurement Science And Technology, 28, 3. doi:10.1088/1361-6501/aa56a7. Abstract
One of the approaches to bypass the problem of electrode polarization in dielectric measurements is the free electrode method. The advantage of this technique is that, the probing electric field in the material is not supplied by contact electrodes, but rather by electromagnetic induction. We have designed an inductive dielectric analyzer based on a sensor comprising two concentric toroidal coils. In this work, we present an analytic derivation of the relationship between the impedance measured by the sensor and the complex dielectric permittivity of the sample. The obtained relationship was successfully employed to measure the dielectric permittivity and conductivity of various alcohols and aqueous salt solutions.
Ivan Popov, Lunev, Ivan , Khamzin, Airat , Greenbaum (Gutina), Anna , Gusev, Yuri , and Feldman, Yuri . 2017. The Low-Temperature Dynamic Crossover In The Dielectric Relaxation Of Ice I-H. Physical Chemistry Chemical Physics, 19, 42, Pp. 28610-28620. doi:10.1039/c7cp05731h. Abstract
Based on the idea of defect migration as the principal mechanism in the dielectric relaxation of ice I-h, the concept of low-temperature dynamic crossover was proposed. It is known that at high temperatures, the diffusion of Bjerrum and ionic defects is high and their movement may be considered to be independent. Simple switching between these two mechanisms leads to a dynamic crossover at similar to 235 K. By introducing coupling between the Bjerrum and ionic defects, it is possible to describe the smooth bend in the relaxation time at low temperatures in ice Ih. However, because the mobility of Bjerrum orientation defects slows down at low temperatures, they may create blockages for proton hopping. The trapping of ionic defects by L-D defects for a long period of time leads to an increase in the relaxation time and causes a low-temperature crossover. This model was validated by experimental dielectric measurements using various temperature protocols.
Daniel Agranovich, Ben Ishai, Paul , Katz, Gil , Bezman, Dror , and Feldman, Yuri . 2017. Microwave Dielectric Spectroscopy Study Of Water Dynamics In Normal And Contaminated Raw Bovine Milk. Colloids And Surfaces B-Biointerfaces, 154, Pp. 391-396. doi:10.1016/j.colsurfb.2017.03.051. Abstract
The role of water in bovine milk is more complicated than that of a background solvent. To understand the interaction between water and the constituents of milk, an extensive dielectric study of the gamma-dispersion of raw bovine milk was carried out over the frequency range 0.1-50 GHz and the interval of temperatures (10 degrees C-42 degrees C). Samples were provided by utilizing an extended donor pool. The results reveal that the temperature dependence of the characteristic relaxation times is described by the Arrhenius law. Furthermore, it conforms to a Meyer-Neldel compensation, whereby the pre-factor of the relaxation times is dependent on the activation energy. This entropy/enthalpy compensation is traced to the interaction between bulk water dynamic clusters and other milk constituents. A statistical correlation between the Somatic Cell Count, a traditional measure of milk quality, and the relaxation times is provided as well, opening new vistas for the industrial classification of milk. (C) 2017 Elsevier B.V. All rights reserved.
Tze Ning Hiew, Huang, Rongying , Popov, Ivan , Feldman, Yuri , and Heng, Paul Wan Sia . 2017. A Study Of Moisture Sorption And Dielectric Processes Of Starch And Sodium Starch Glycolate. Pharmaceutical Research, 34, 12, Pp. 2675-2688. doi:10.1007/s11095-017-2252-x. Abstract
This study explored the potential of combining the use of moisture sorption isotherms and dielectric relaxation profiles of starch and sodium starch glycolate (SSG) to probe the location of moisture in dried and hydrated samples. Starch and SSG samples, dried and hydrated, were prepared. For hydrated samples, their moisture contents were determined. The samples were probed by dielectric spectroscopy using a frequency band of 0.1 Hz to 1 MHz to investigate their moisture-related relaxation profiles. The moisture sorption and desorption isotherms of starch and SSG were generated using a vapor sorption analyzer, and modeled using the Guggenheim-Anderson-de Boer equation. A clear high frequency relaxation process was detected in both dried and hydrated starches, while for dried starch, an additional slower low frequency process was also detected. The high frequency relaxation processes in hydrated and dried starches were assigned to the coupled starch-hydrated water relaxation. The low frequency relaxation in dried starch was attributed to the local chain motions of the starch backbone. No relaxation process associated with water was detected in both hydrated and dried SSG within the frequency and temperature range used in this study. The moisture sorption isotherms of SSG suggest the presence of high energy free water, which could have masked the relaxation process of the bound water during dielectric measurements. The combined study of moisture sorption isotherms and dielectric spectroscopy was shown to be beneficial and complementary in probing the effects of moisture on the relaxation processes of starch and SSG.
2016
Evgeniya Levy, Barshtein, Gregory , Livshits, Leonid , Ben Ishai, Paul , and Feldman, Yuri . 2016. Dielectric Response Of Cytoplasmic Water And Its Connection To The Vitality Of Human Red Blood Cells: I. Glucose Concentration Influence. Journal Of Physical Chemistry B, 120, 39, Pp. 10214-10220. doi:10.1021/acs.jpcb.6b06996. Abstract
The vitality of red blood cells depends on the process control of glucose homeostasis, including the membrane's ability to ``switch off'' D-glucose uptake at the physiologically specific concentration of 10-12 mM. We present a comprehensive study of human erythrocytes suspended in buffer solutions with varying concentrations of D-glucose at room temperature, using microwave dielectric spectroscopy (0.5 GHz-50 GHz) and cell deformability characterization (the Elongation ratio). By use of mixture formulas the contribution of the cytoplasm to the dielectric spectra was isolated. It reveals a strong dependence on the concentration of buffer D-glucose. Tellingly, the concentration 10-12 mM is revealed as a critical point in the behavior. The dielectric response of cytoplasm depends on dipole-matrix interactions between water structures and moieties, like ATP, produced during glycolysis. Subsequently, it is a marker of cellular health. One would hope that this mechanism could provide a new vista on noninvasive glucose monitoring.